Move fast. Miss nothing.
With over 7 million plasmids sequenced since 2021, Plasmidsaurus has been the established expert in long-read whole plasmid sequencing, known for consistently delivering same-day or overnight results. But unlike other providers, we deliver fast results without making any concessions on what matters most.
Higher accuracy. Fewer errors. Every Plasmid.
Better chemistry, deeper coverage, and advanced bioinformatics for stronger performance across every plasmid, whether routine, low-concentration, or complex.
Speed you can actually count on.
With 10 lab locations and 1300+ dropboxes globally, get results overnight, or even same-day in some locations.
Reports that give you confidence.
Every result includes an interactive plasmid map to spot and confirm differences, plus exportable publication-ready figures.
How it works
Send us your sample in the way that’s most convenient for you. Colony, overnight culture, or purified plasmid. Sizes up to 300kb. Concentrations as low as 20 ng/µL. Get a report back in your inbox by the next day!
Zero hassle. Zero time wasted. Zero PCR. ZeroPrep.
Skip the miniprep and speed up your experiments. Send a plate, liquid culture, or colony and get full-length, amplification-free plasmid sequencing overnight or even same day.
Ditch minipreps for less than a cup of coffee.
Tired of performing minipreps on every single sample? ZeroPrep can eliminate the headache and hands-on burden and save your lab significant time on up-front prep, allowing you to view your plasmid sequences first and only miniprep the hits you want.
Get to the results you want faster.
Other providers add days to your timeline when they handle sample prep. We don't. Drop off your raw colonies and get your results overnight or same day, speeding up your experiment by up to a day compared to purifying every plasmid.
PCR can hide what's really in your plasmid. ZeroPrep doesn't.
Others use amplification, which introduces bias, errors, and dropouts in GC-rich or repetitive regions. ZeroPrep is PCR-free, which reduces the risk of missing unexpected inserts, alternate origins, and structural variants the others miss.
The sequencing partner that serious labs rely on
Trusted by scientists everywhere, including:
- AddGene, the world's largest plasmid repository, for plasmid quality control (QC) sequencing.
- All 50 top NIH-funded institutions
- ~99% of NCI-designated cancer centers
- ~95% of US R1 research universities
- Used by all 10 top biopharma
Join Over 70,000 Scientists
Accelerate your research with Plasmidsaurus. What would you do with more time?
Put some plasmids in the dropbox at 3pm yesterday and got the results at 02:55am. Less than 12 hours for the full annotated sequence of 4 plasmids is quite mind boggling.
This $15 in sequencing saved hundreds in materials and months of time.
Without Plasmidsaurus we couldn’t confidently sequence our constructs. It has been a game changer for us, saving us both time and money.
The most significant improvement Plasmidsaurus provides over competitors is their failure rate was pretty high while Plasmidsaurus’ approached zero.
We’ve been sequencing with Plasmidsaurus to validate clones. It’s faster and more comprehensive than ordering cohorts of Sanger primers.
Shoutout to @Plasmidsaurus for not only sequencing my 12 samples in less than a day but also being able to identify stuff like entire plasmid duplications!
Plasmidsaurus identified backbone errors in plasmids cloned by our gene synthesis company, which we could never have identified via Sanger.
Intuitive, interactive results.
We don't just sequence your plasmid. We help you understand it.
Get confidence in every base
See basecalls for each position across all sequencing reads.
Zero in on mutations, insertions, and deletions
Catch errors quickly with base-level insights and amino acid consequences.
Results in your inbox
View overnight results quickly before diving in for deeper analysis.
Try out our live demo with real data
See what Sanger is missing
Verify plasmid identity, purity, and stability.
Data deliverables & bioinformatics
Consensus sequence (.fasta, .gbk)
Plasmid map (.html)
Read length histogram (.png)
Virtual gel (.png)
Chromatogram (.ab1, .gbk)
Coverage plot (.png, .gbk)
Per-base data (.txt, .tsv)
<ul>
<li>SAMPLE.tsv: Indicates how well the raw reads agree with the consensus sequence at each position. </li>
<li>SAMPLE_multimer_analysis.txt: Indicates the % distribution of the various concatemer forms of the consensus sequence (monomer, dimer, trimer, etc.)</li>
<li>SAMPLE_summary.tsv: Indicates the length, average coverage, relative composition (by moles and mass), total reads, total bases, and % E. coli genomic DNA contamination for the consensus sequence.</li>
</ul>
Raw read sequenced (.fastq.gz)
Product specs & service levels
| Service Level | Size | Concentration | Volume | Purity | Cost | Target Turnaround Time |
|---|---|---|---|---|---|---|
| Standard Low Concentration | <25 kb | 20 - 200 ng/µL | 10 µL | Column or bead purified | $15 | 1 day |
| Standard High Concentration | <25 kb | 200 - 1000 ng/µL | 4 µL | $15 | ||
| Big | 25 - 125 kb | 50 - 400 ng/µL | 20 µL | $30 | ||
| Huge | 125 - 300kb | 50 - 400 ng/µL | 40 µL | $60 |
| Service Level | Sample Input | Plasmid Size | Cost | Target Turnaround Time |
|---|---|---|---|---|
| Liquid Culture | 20 μL - 100 μL of turbid culture in LB media, OD600 > 0.3 (recommended) Liquid should appear cloudy upon submission | <20 kb | $20 | 1 day |
| Colony Resuspension | >2 mm colony resuspended in 20 μL of LB media (recommended) Liquid should appear cloudy upon submission | |||
| Agar Plate Colony | ~1 cm2 patches clearly labeled OR >2 mm colonies clearly circled and labeled |
Ready to sequence?
Send us extracted and purified plasmid DNA, or go straight from colony to sequence.
Relevant resources
Related products
Bacterial WGS
Want to sequence an entire genome? Use Whole Genome Sequencing for nearly any organism.
Amplicon
Need to verify cloning and gene editing with a specific region? Try Amplicon Sequencing.
Custom
Have sequences that are really small or really big? Have large plasmid variant libraries? Contact us via Custom Sequencing services for more options.
FAQs
Plasmidsaurus Whole Plasmid Sequencing is performed using the newest long-read sequencing chemistry from Oxford Nanopore Technologies (ONT).
We use a transposome complex to cut your circular plasmid at a random point, creating an amplification-free library of full-length linear molecules, so that each sequencing read spans the entirety of the plasmid. We return the full length consensus sequence of your plasmid, beginning at the origin.
We do not guarantee a specific level of coverage as the number of raw reads generated can vary due to length and sample quality, though typically samples yield hundreds of raw sequencing reads. These go through multiple quality filtering steps to ensure we are delivering the best possible assembly.
We use the latest flowcells and chemistry kits from Oxford Nanopore, along with the latest Super Accurate basecalling model. The vast majorities of our plasmid assemblies contain no errors compared to the reference, which you can read more about here. Consensus accuracy is often above Q60, which corresponds to 99.9999%, or one error per 1,000,000 bases.
We do not guarantee a specific level of coverage as the number of raw reads generated can vary due to length and sample quality, though typically samples yield thousands of raw sequencing reads. These go through multiple quality filtering steps to ensure we are delivering the best possible assembly.
Average coverage is reported in the coverage plot file, and coverage over ~20x indicates a very accurate consensus.
This service is intended for a clonal population of molecules. If your species are very similar (e.g. differ by only a few nucleotides), the pipeline will most likely create a single consensus file, with mixed peaks observed in the .ab1 file where there are SNPs and indels.
If your species are sufficiently distinct in size or sequence, the pipeline will generate a single consensus sequence for the molecular species that produces the largest amounts of total sequencing data. (Please note that multimer forms such as dimers, trimers, etc. are not considered different molecular species by the pipeline, so you will only receive the monomer consensus sequence by default).
Ultimately, which species ends up producing a consensus will vary depending on overall sample quality, coverage, and relative abundance of each species. Sequencing is considered successful if the pipeline is able to generate a consensus, even if it is not your target.
Re-sequencing mixtures won't change the relative proportions of the species (and hence which species generate a consensus), but you can submit multiple aliquots if you need higher overall coverage. Custom sequencing is available to sequence mixed populations (e.g. large barcode or variant libraries). You can submit requests for custom projects via the Custom sequencing page.