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Sequence everything.

Whole Plasmids | Amplicons | Microbes | Colonies | Anything

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Just received results whole plasmid sequencing from @plasmidsaurus. Amazing service with very cheap price. Highly recommended! Shoutout to @plasmidsaurus for recently returning me sequencing results for three plasmids. Excellent job! Shout out to @plasmidsaurus for providing plasmid sequencing to @smithcollege intro Bio labs. Thanks for supporting historically excluded people in STEM! I tried @plasmidsaurus for sequencing of several 10kb plasmid. Very fast, convenient. Just for 15 $. it's worth it. Marry Christmas from @plasmidsaurus ❤️❤️❤️ all 18 of my plasmid DNA successfully cloned!! Just used @plasmidsaurus for the first time, $15 the whole plasmid sequencing. I highly recommend. It's just amazing. We started sending our samples recently and got excellent results. I highly recommend trying it. I used @plasmidsaurus to sequence my plasmids this week... And guess what! It was FANTASTIC! Easy, fast and cheap! Way better than doing 10 Sanger sequencing with primers! I absolutely love @plasmidsaurus A total game-changer when it comes to cloning. @plasmidsaurus Can someone explain to me why your full length reads are so cheap compared to other vendors? I'm talking an order of magnitude or more. It seems too good to be true... Love the Dinocoins @plasmidsaurus. So much easier to get orders placed. Nice work! Had the most wonderful experience with @plasmidsaurus. Got my entire plasmid sequenced in just 5 days and at nearly the cost of a Trio at McDonalds 🤩it’s just $15. The precision and accuracy are wonderful. Must try if you haven’t. @plasmidsaurus has truly revolutionized how everyone does molecular cloning! Excellent sequencing results from the first shipment of plasmids to @plamidsaurus We want one dropbox @CNB_CSIC ASAP!! A bit late to the party, but @plasmidsaurus is 🔥 🔥, cannot recommend enough! YES! A month ago, we used @plasmidsaurus for the first time and were able to explain two months of super-confusing experimental results🤩. The data we got back were easy to use, annotated plasmid maps. Our new samples are on their way now from this brand new dropbox! The fact that I can give @plasmidsaurus a few bucks and they'll tell me exactly what went wrong is pretty magical (: the best business in biotech Exciting sequencing data from @plasmidsaurus! It's time for wine & assembly! We are using them happily as well - despite the long - distance shipping. Any chance @plasmidsaurus that you want to open a branch in Switzerland? Just got back my first genome sequence from @plasmidsaurus and everything looks beautiful! Excited to look further into my #rhizobia#genomes and play around with nanopore data! Another flawless sequencing from @plasmidsaurus that makes me feel like I will never ever learn to clone stuff. With Sanger it would have been another two weeks of head scratching and frustration. I highly recommend using #plamidsaurus for sequencing of your plamids/amplicons! No primers required and awesome results! @plasmidsaurus made my day! It’s holiday week, the result usually come on Monday, but it didn’t come until this morning. I literally checked on my account 4+ times a day just for this moment 🥹. Happy thanksgiving!!! I need to say again that their work is amazing!!! I ordered sequencing 3 times in this week😂 I always put my samples in drop boxes at our building, then receive results tomorrow morning! Can't believe that @plasmidsaurusdidn't exist when I was in grad school. I now find myself doing free PR for them because they're so awesome 🧬 🧬 🧬 Drafting posters to put up everywhere... Thank you to @plasmidsaurus for full plasmid sequencing so I can stop using 5 primers just to sanger sequence just the ORF of my plasmid... Plasmidsaurus rocks 🤙 Thank you @plasmidsaurus🙏🏼🙏🏼.. You constantly reduce my stress in grad school! Plasmidsaurus rocks! @plasmidsaurus Rocks. Thanks @plasmidsaurus for helping set this dropbox up - for researchers in the precinct this means you get FREE sample shipping to the US, for all your plasmid sequencing needs with @plasmidsaurus!! @PeterMacRes @Haem_pmcc_rmh @PeterMacCoE @WEHI_research @UMCCR @VCCCAlliance Ok, I know I am super late to this party, but I just had my first @plasmidsaurus results returned to me and am absolutely blown away! Such a great service if you need to sequence larger parts of your plasmid. I can’t believe I only just learned about @plasmidsaurus… Years of struggling with multiple Sangar reads on countless constructs - I send them a plasmid and I have the ENTIRE sequence two days later for cheaper than Sangar would be! Thank you @plasmidsaurus 🦖🦕 😁 I am getting frustrated. For years, I have amplified 16S rRNA sequences by PCR, purified the amplicons, and sent them out for sequencing with no trouble. But other amplicons, where I need to send primers, are a mess. I am ready to just clone them and submit to @plasmidsaurus Don’t even need to clone them, can just send the PCR products and they’ll sequence. No primers needed I'm officialy impress with @plasmidsaurus!!! Really great service! It would be great to have a dropbox here in 🇲🇽. For now, our plasmid results look awesome and eventually will prove other products. Thanks!! 🦖🦕 They are really awesome and do a great job with long amplicons too! No more primer walking with Sanger. Just got my first set of sequencing results for recombinant plasmids from @plasmidsaurus I’m so impressed at the speed and accuracy of the results! So happy to know about this company! The Blind Lab LOVES @plasmidsaurus! Cheap ion-torrent sequencing of entire plasmids!!!! Awesome news! If you haven't tried @plasmidsaurus yet, I highly recommend. 😃🦖🦕 Had a very satisfying Monday checking my results from @plasmidsaurus confirming my plasmid obtained from 4-pieces assembly 👍🏻💪🏻😎🧬 Awesome. This is way more convenient! Very impressed with the @nanopore bacterial genome sequencing service from @plasmidsaurus, got analyzed genome data back within a week after sample pickup from the dropbox and it looks good! I spent hours today looking at sequencing data and invoices and notebooks trying to figure out why a set of plasmids had erroneous vectors… and then I found the tell tale mistake 15 messages into an email threa that was forwarded to me after the fact in 2020. All I’m saying is, sequence your full plasmid EVERY TIME (wouldn’t have caught it without @plasmidsaurus ) and if you need a private investigator, I’m your gal. YES THIS IS WONDERFULLY TIMED - the bacterial genome I am working with is like a zillion scaffolds from 454 pyro… I can’t trust it! But I *do* trust Plasmidsaurus ❤️ it is extremely helpful! helped me figure out a restriction site was no longer present in a plasmid we had received from another lab! @plasmidsaurus sequencing results came back and I'm happy to report that I can proceed with the next steps on this experiment. 😅😭 Another day, another cloning project saved by @plasmidsaurus. The plasmid my friend was given was supposed to be 11kb… PCR results were weird. Wouldn’t you know it had deleted bits of itself and only 2k was left! It happened to me: discovered concatamers in a plasmid prep thanks to @plasmidsaurus Hmm, this is supposed to be a 5.3 kb plasmid. Cloning failures really starting to make sense now. Somehow dumped the LacI CDS, yikes! Hilariously, will still express protein, just totally unregulated.🙃 Thanks @plasmidsaurus! why are all my plasmids concatemers?! 😂😭 dangit @plasmidsaurus i was happier before i knew Plasmid dimers! Grown from a single colony streaked from an addgene stab. Thanks @plasmidsaurus! Such an easy way to analyze plasmids before use. (Ab)Using the @plasmidsaurus sequencing service is sort of amazing. For $30!!!, I was able to rule out the presence of XX/X0, ZZ/ZW, and ZZ/Z0 chromosomal sex determination in the sea urchin I work with, and was able to generate some hypotheses to follow up on. Solid business model of @plasmidsaurus to show me that 1/3 of my plasmid is missing so now I send all of my constructs to them for sequencing Probably going in the thesis acknowledgements someday for that one Shoutout to @plasmidsaurus for recently returning me sequencing results for a plasmid containing 1.1kb stretch of TTAGGG telomeric repeats - which previously resisted all attempts at sequencing! And for USD15! My plasmid was giving unexpected restriction patterns. Thanks to @plasmidsaurus, I realized there was a bacterial insertion between the KanR gene and the replication origin. Apparently, this is a common phenomenon in some plasmids (eg. pCambia-based)🤯 Just dropped off some plasmids in the @plasmidsaurus dropbox and it was getting full. Glad to see it! Hopefully that keeps our dropbox in business. Because of them, I *know* things about our plasmids. Deep secrets once shrouded in kb of mystery, now laid bare. Life changing. @plasmidsaurus now sequences bacterial genomes: Everytime I receive results from @plasmidsaurus@grnfluoresceblg 😂 Sequencing results on the weekend 🥳 Thanks @plasmidsaurus for the quick service! Found a treasure in @plasmidsaurus dropbox Mfw i see a new lab investigating our @plasmidsaurus dropbox Me: They offer $15 rapid (sometimes same day!) whole plasmid sequencing (+more). @plasmidsaurus has reached their peak. Plasmidsaurus:
I honestly have you guys sequence EVERYTHING before I use it now - even stuff I pull out of my own freezer. Amazing how much stuff from my own freezer has duplications... — James West PhD (@James_West_PhD), Mar 4, 2024

1 in 25 samples has unexpected dimers

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Verify new samples
QA lab work before errors can multiply
Unexpected results? Find out exactly what went wrong.
Check sample purity before expensive runs or trials
Verify before you publish or ship
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No primers

Say goodbye to oligo synthesis costs and delays

Fastest results

Drop off as you leave and have data waiting in the morning

Unreal value

Peace of mind starting at $15

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Verify and document your sample’s identity, purity, and stability.

See what your Sanger sequencing missed with our whole-plasmid sequencing and pLannotate gene map.
  • Resolve repetitive regions that stymie traditional sequencing, e.g. ITRs in AAVs.
  • Identify structural issues like duplications, deletions, and rearrangements.
  • Reveal richer information about mixed populations and unstable elements that are invisible to Sanger sequencing.
A histogram with one dominant peak indicates a clean prep with a single plasmid.
A histogram with multiple peaks indicates unexpected products, deletions, recombinations, or concatemers.

Why use Plasmidsaurus?

We pioneered whole-plasmid sequencing and continue to innovate at the cutting edge of sequencing technology.

start sequencing
Sequence plasmids.

Full plasmid sequence. Overnight. No primers required.

Fully sequence your entire plasmid, including your insert and backbone, in one run. No primers are used: say goodbye to primer walking and oligo synthesis costs and delays. Perfect for repetitive sequences including AAV LTR, ITR, and GC-rich repeats.

Sequence amplicons.

Full-length coverage of PCR products, with no primers.

Use our standard amplicon service to quickly confirm clonal amplicons, or our Premium PCR service for deeper, end-to-end sequencing of mixed populations. No primers are used, even for long amplicons, eliminating the need for oligo synthesis and its cost, effort, lost time, and failure risk.

Sequence microbes.

Sequence and assemble clonal populations of bacteria or yeast.

Receive a complete, high-accuracy genome assembly in one day -- weeks faster than previously possible. We return a fully annotated genome assembly along with the raw sequencing data.

Sequence colonies.

ZeroPrep: skip the miniprep and reclaim your time.

Our groundbreaking ZeroPrep service eliminates the time and expense of manual minipreps. Send us liquid cultures or colonies on agar plates and only move forward with the clones that count. For a scant premium over the cost of a miniprep kit, ZeroPrep is a zero-brainer.

Sequence anything.

Custom Projects.

Got a protein engineering library that needs deep characterization? An unknown mixture? Need lots of data? We can sequence it to your specifications with long reads and 1-week turnaround for an affordable price.