Eukaryotic Genome Sequencing with Extraction
Service Level | Size | Number of Cells | Preservative | Cost |
---|---|---|---|---|
1 Gb data target | 20 - 60 Mb | 1 x 10^6 | 200 µL Zymo Shield | $300 |
5 Gb data target | 75 - 250 Mb | $550 | ||
15 Gb data target | 300 - 750 Mb | 2 x 10^6 | $1050 | |
Full flow cell (50 - 100 Gb data target) | 1 - 3.3 Gb | $1800 |
Count your cells.
This service requires 1-2 x 10^6 cells (depending on the service level) that are preserved in Zymo 1X DNA/RNA Shield. This service is available for most cultured or disassociated eukaryotic cell types that do not have a cell wall (including isolated protoplasts with their cell walls removed). We accept cell pellets from both BSL1 and BSL2 strains. If you have fewer cells available than listed above, we can often work with lower inputs too - please email us at support@plasmidsaurus.com to discuss options.
We do not currently accept blood from any species, nor any intact tissues or organs from animals, plants, insects, fungi, etc. We also do not accept cells preserved by other methods such as flash freezing, ethanol, desiccation, RNAlater, etc. If you’d like to sequence these alternative sample types and preservation methods, please perform extraction on your end and ship us only the purified gDNA for sequencing.
Wash and pellet cells.
Pellet the cells by centrifugation and remove as much supernatant as possible.
Resuspend the pellet in 1 mL PBS to wash, then pellet again and remove supernatant.
Resuspend the pellet.
Resuspend the final pellet in 200 µL of Zymo 1X DNA/RNA Shield in a 200 μL strip tube.
Please do not use any other type of preservation media, as they do not preserve the DNA as effectively and do not fully inactivate the host cells, which is required for safe handling in our lab.
Label your tubes or plates.
- Set up your order online and receive your unique 6-character order ID.
- Write the first 2 characters of your order ID on the 1st tube of each strip
- Set up your order online and receive your unique 6-character order ID. On the order page, please check the “Is this a plate submission?” checkbox, then select if your plate is numbered sequentially by column or by row.
- Label the front edge of the plate with the full 6-character order ID.
- If you are using column numbering, label the first column (i.e., samples 1 to 8). If you are using row numbering, label the first row (i.e., samples 1 to 12).
Load and package your sample.
Add the correct volume of your sample(s) from the table above. Make sure to seal and package your samples well to make sure they arrive safely for sequencing!
- Ensure caps fit snugly and close them firmly. Do not use brands of strip tubes where the caps can easily pop open during shipping.
- Place the labeled tubes into a small protective vessel (such as a Falcon tube, a small box, or bubble wrap) to protect them during shipping. Why?
Start with well A1 and load your samples contiguously. Our preference is for samples to be loaded by column but we can accept samples loaded by row if indicated in the order. Do not leave any empty wells between samples.
- Ensure your plates are sealed extremely thoroughly to survive shipping without leaking — sadly we receive a lot of leaky plates. We recommend using a heat seal (rather than adhesive) or snugly-fitting strip caps on top of the plate. If you only have adhesive options, click here for some examples of sealing technique.
- To prevent cross contamination of samples, our customers have also had good success with dehydrating plates: spin the plate(s) down and then place the plate(s) on a 65 degree heating block for 0.5 to 1 hour or until dry. After dry, you can seal the plate with sticky film to prevent contamination of the sample during transport. We will rehydrate the sample in our lab for sequencing.
- Wrap each plate individually in bubble wrap or other packaging material to prevent cracking and seal punctures. DO NOT stack multiple plates directly on top of each other, as the wells of one plate will likely puncture the seal of the next plate.
Dropoff or ship.
Print out your order form. Fold so that QR code is readily visible and place in a small bag with your samples.
For fastest results and easiest shipping, place your samples in a Plasmidsaurus Dropbox!
If you ship your samples to us directly:
- Place packaged samples in a sturdy cardboard or padded envelope or a cardboard box. If reusing old packaging, ensure that any hazard warnings are completely obliterated. Multiple orders can be shipped in the same package, as long as each order is placed into a separate bag with its corresponding order confirmation sheet.
- Do not add ice packs or dry ice. Samples should be shipped at room temperature.
- Ship via any Express or First-Class shipping carrier such as FedEx, UPS, DHL, or USPS. The shipping address will be provided on your order confirmation sheet.
Please reach out to support@plasmidsaurus.com for specific guidance on international shipping, since the types of eukaryotic samples can vary greatly.