Microbiome Sequencing

Get a snapshot of your microbial communities, overnight.

16 S 1

Rethink your microbiome workflow

Most microbiome studies are still built around large batch sequencing runs with slow turnaround times and workflows that rely on older short-read technologies. Plasmidsaurus Microbiome Sequencing offers a new model: full-length 16S, 18S, or ITS reads with more precise species-level classification, delivered as quickly as overnight.

 

Designed for speed, precision, and flexibility, Plasmidsaurus microbiome sequencing services are ideal for monitoring environmental and synthetic communities, understanding an experimental biome and how it responds to stimuli, and finding new species in wild communities. Send us raw biological samples, genomic DNA, or purified amplicons and quickly receive full-length reads and species-level classification.

Microbiome 1

Confirm what's in your community

Confirm your system has the expected species diversity before starting your experiment, with insights direct from raw samples, genomic DNA, or PCR amplicons.

Rapid results

Monitor with rapid results

Easily monitor your microbial communities over time, across locations, and in response to stimuli, with full length reads with taxonomic classification in under one day.

Microbiome 3

Discover with species-level clarity

Precisely identify microbial species that short-read sequencing can’t resolve. Discover what species are present and in what relative abundances.

Which sample input is right for me?

Microbiome 16S Amplification & Sequencing with DNA Extraction

Sample input

Raw samples (including soil, feces, swabs, and more)

Ideal for

Exploring the bacteria and archaea composition of a wide variety of samples

Service levels

Standard: Up to 5,000 reads
Big: Up to 10,000 reads
Huge: Up to 20,000 reads
Bronto: Up to 500,000 reads

As fast as

1 - 3 days

Availability

Within the US

As low as

$60

Microbiome 16S Amplification & Sequencing

Sample input

Extracted environmental DNA

Ideal for

Samples requiring specialized extraction protocols that we don’t support or labs based outside the US

Service levels

Standard: Up to 5,000 reads
Big: Up to 10,000 reads
Huge: Up to 20,000 reads
Bronto: Up to 500,000 reads

As fast as

Next day

Availability

Globally

As low as

$45

Microbiome Amplicon Sequencing

Sample input

Purified amplicons of full-length 16S, 18S or ITS genes, or a mixture thereof

Ideal for

Studying fungi, protists, algae or other microbes that lack 16S genes and are commonly found in soil and water samples

Service levels

Standard: Up to 5,000 reads
Big: Up to 10,000 reads
Huge: Up to 20,000 reads
Add cleanup for $5

As fast as

Next day

Availability

Globally

As low as

$30

Product specs & service levels

All samples are sequenced with ONT.

Service LevelSample InputRead Depth/SampleCostTarget Turnaround Time
StandardEnvironmental samplesUp to 5,000$603 business days
BigUp to 10,000$90
HugeUp to 20,000$150
BrontoUp to 500,000$265

All samples are sequenced with ONT.

Service LevelSample InputRead Depth/SampleMinimum VolumeConcentrationCostTarget Turnaround Time
StandardgDNAUp to 5,00010 µL min10 ng/µL$451 business day
BigUp to 10,00020 µL min$75
HugeUp to 20,00040 µL min$135
BrontoUp to 500,00040 µL min$250

All samples are sequenced with ONT. Add cleanup for $5 while placing your order. Cleanup adds 2 days to expected turnaround times.

Service LevelSample InputRead Depth/SampleMinimum VolumeConcentrationCostTarget Turnaround Time
StandardLinear double-stranded DNAUp to 5,00010 µL min10-20 ng/µL$301 business day
BigUp to 10,00020 µL min$60
HugeUp to 20,00040 µL min$120

Data deliverables & bioinformatics

Species and genus diversity

Image 12
Relative abundance of the top 20 species and top 20 genera in your sample

Taxonomic diversity

Image 14
Table of relative abundance of taxa identified

Read identifications

Image 11
Table of taxa to which each read was assigned

Statistics

QWPJT4 7 ZMC 25 1 histogram
Summary of number of reads, distribution of lengths, total bases, read quality scores.

Raw reads

Frame 1171276161
All raw sequencing reads from your sample provided in fastq

Ready to sequence?

Send your messy sample, purified DNA, or PCR amplicon. We'll handle the rest.

Microbial Amplicon

FAQs

We sequence each sample the newest long-read sequencing technology from Oxford Nanopore Technologies (ONT), including the following components:

  • Constructing long-read sequencing library using the newest v14 library prep chemistry.
    • For the Microbiome 16S Amplification & Sequencing service, this includes barcoded full-length amplification of the 16S gene with our in-house sequencing primers.
    • For the Microbiome Amplicon Sequencing service, this includes end-ligation of your 16S, 18S, and/or ITS amplicons. We do not further amplify your amplicons.
  • Sequencing the library with a primer-free protocol using the most accurate R10.4.1 flow cells (raw data is delivered in .fastq.gz format), producing a full-length sequencing read for each amplicon.

As per Oxford Nanopore's specs for the chemistry and flowcells we currently use for Microbiome Sequencing, the raw read accuracy is typically >99.5%. We basecall the raw reads using ONT’s super-accurate basecalling model. We require a minimum raw read Qscore of 10 (90% accuracy) during sequencing.

We generate metrics on the relative abundance of all species that comprise at least 0.1% of the microbial community.

DNA/RNA Shield-preserved source materials for extraction cannot be shipped via your local US dropbox, but instead must be shipped directly to our Eugene lab. You can request a free shipping label during order submission or ship on your own to:

Plasmidsaurus
1850 Millrace Drive, Suite 200
Eugene, OR 97403

If you would like us to attempt metagenomic DNA extraction from other types of materials that are not listed here, please contact us at support@plasmidsaurus.com to discuss options before order submission.