Whole Genome Sequencing

Fast and complete genome sequences for nearly any organism.

Bacterial Genome3

Plasmidsaurus speed at genome scale

Most whole genome sequencing still relies on short reads, slow turnaround times, and fragmented assemblies that miss the bigger picture. Plasmidsaurus Whole Genome Sequencing lets you go straight from cells to complete genome assemblies in as little as a day, bypassing the need for tricky sample prep or complex bioinformatics pipelines. With Plasmidsaurus convenience and speed, you can discover new biology, design new pathways, and monitor your experiments with the full picture.

Fast answers, full coverage

Fast and Complete

Complete coverage

Achieve complete, gapless genome assemblies with de novo assembled long reads — capturing regions and structural features that short-read assemblies leave fragmented or unresolved

Sequencing Results Ready

Maximal convenience

Start from cells and get assembled genomes. No need to master new sample prep or bioinformatics techniques—spend time interpreting results and leave the rest to us.

Send In cell Pellets

Rapid results

Get high-accuracy genome assemblies from extracted DNA within days.

Which organism are you working with?

Bacterial Genome Sequencing

Organism

Cultured bacterial and archaeal isolates with genomes up to 12 Mb

Sample input

Cell pellets preserved in Zymo DNA/RNA Shield or extracted gDNA

DNA extraction available for

All BSL1 and BSL2 strains

As fast as

1 - 2 business days.
See service level details below for more info.

As low as

$90

Yeast Genome Sequencing

Organism

Cultured budding or fission yeast isolates with genomes up to 20 Mb

Sample input

Cell pellets preserved in Zymo DNA/RNA Shield or extracted gDNA

DNA extraction available for

18 genera of yeast

As fast as

1 - 2 business days
See service level details below for more info.

As low as

$150

Eukaryotic Genome Sequencing

Organism

Organisms with genomes from 20 Mb to 3.3 Gb including protozoans, plants, animals, and humans

Sample input

Cell pellets preserved in Zymo DNA/RNA Shield or extracted gDNA

DNA extraction available for

Most organisms and cell types without a cell wall, including human tissues
Questions? Contact us

As fast as

3 - 6 business days.
See service level details below for more info.

As low as

$250

Rethink your genome sequencing workflow

Bioprospecting

Get de novo assemblies of genomes from novel isolates or wild organisms for functional characterization. Discover new enzymes, proteins, or pathways with potential industrial or biomedical applications.

Directed evolution

Quickly and reliably identify complex mutations that lead to desirable phenotypes, including rearrangements, duplications, insertions, and integration events that short-read or targeted approaches often miss.

Strain engineering

Sequence periodically throughout a strain editing campaign to establish baseline truth, verify that intended genome edits were successful, identify off-target modifications, and validate prior to production.

Structural variant detection

Accurately detect a wide variety of structural variants that are missed by short read platforms.

Integration mapping

Characterize integration sites after genome editing, insertional mutagenesis, or retroviral infection

Novel genome assembly

Discover the genomic architecture of new organisms with de novo genome assembly.

Need ultra-precise SNP detection? Choose hybrid!

Our optional Hybrid services supplement your Oxford Nanopore-based data with Illumina reads (2 x 150bp paired-end sequencing) for polishing errors in tricky homopolymers and methylated motifs. Our Illumina data targets (not guaranteed) are:

 

  • Bacteria Hybrid: 100 Mb
  • Bacteria Big Hybrid: 170 Mb
  • Yeast Hybrid: 290 Mb

 

Not available for Eukaryotic Genome Sequencing.

Choose Hybrid
Thumbs up

"Nothing feels quite like "We live in the future" more than getting back sequenced and assembled bacterial genomes in less than 24 hr. Thanks Plasmidsaurus!"

Morgan Carter
Assistant Professor at UNC Charlotte

Product specs & service levels

  • Add $15 and 1-2 business days to turnaround time for DNA extraction
  • We support extraction from a single isolate of any BSL1 or BSL2, gram + or - bacterial species.
     
Service LevelSizeSequencing PlatformSample Submission from gDNASample Submission from CellsCost Target Turnaround Time 
Standard<7 MbONT20 μL at 50 ng/μL normalized concentration4-6 x 10^9 cells in 500 µL Zymo 1X DNA/RNA Shield$901  business day
Big7-12 Mb$105
Hybrid<7 MbONT + Illumina30 μL at 50 ng/μL normalized concentration$1656-8 business days
Big Hybrid7-12 Mb$195
  • Add $15 and 1-2 business days to turnaround time for DNA extraction
  • We support extraction from the following host types: Asbya, Kluyveromyces, Candida, Lipomyces, Debaryomyces, Metschikowia, Eremothecium, Pichia/Komagataella, Endomyces, Saccharomyces, Hansenula, Saccharomycopsis, Hanseniaspora, Schizosaccharomyces, Issatchenkia, Torulopsis, Kloekera, Yarrowia.
     
Service LevelSizeSequencing PlatformSample Submission from gDNASample Submission from CellsCostTarget Turnaround Time
Standard<20 MbONT20 μL at 50 ng/μL normalized concentration50 - 100 mg cell pellet in 500 µL Zymo Shield$1501 business day
Hybrid<20 MbONT + Illumina30 μL at 50 ng/μL normalized concentration$2556-8 business days
  • Add $50 and 2-3 business days to turnaround time for DNA extraction
  • We support extraction from most organisms and cell types without a cell wall, including human tissues. Question about whether we can extract your sample? Contact Support.
  • How do I determine my data target? Read this FAQ here!
  • If you have fewer cells available than we require in the table below, we can often work with lower inputs too - please email us at support@plasmidsaurus.com to discuss options.
     
Service LevelGenome Size Range (30X Coverage)Sequencing PlatformSample Submission from gDNASample Submission from Cells CostTarget Turnaround Time
1 Gb data target20-60 MbONT20 μL at 50 ng/μL normalized concentration1 x 10^6 cells in 200 µL Zymo Shield$2503-5 business days
5 Gb data target75-250 Mb40 μL at 50 ng/μL normalized concentration$500
15 Gb data target300-750 Mb60 μL at 50 ng/μL normalized concentration2 x 10^6 cells in 200 µL Zymo Shield$10005-8 business days for FASTQ data; 1-4 weeks for assemblies
Full flow cell (50-100 Gb data target)1 - 3.3 Gb60 μL at 50 ng/μL normalized concentration$1750

Looking for more data? Working with an organism with a bigger genome?

Check out our custom sequencing solutions to see how we can help, from running full Illumina flow cells to custom ONT library prep and more.

Custom2

Data deliverables & bioinformatics

Genome reference assembly

Reference Assembly
Full assembled sequence of the genome (may contain multiple contigs for the chromosomes and extrachromosomal elements)

Functional annotation

Fuctional Annotation
Complete set of annotated features with gene products and functional assignments

Assembly statistics

Genome Assembly statistics
Assembly statistics including total genome size, number of contigs, genome coverage, and completeness

Sequencing statistics

Genome Sequencing Statistics
Sequencing statistics including the number, length, and quality of individual raw reads

Raw reads

Genome Raw Reads
All raw reads from your sample are provided in fastq format

Assembled contig ID (for bacteria only)

Assembled contig ID (for bacteria only)
Identifies the species of each assembled contig. Only available for bacteria (yeast and eukaryote are coming soon!)

Interactive genome alignment (for bacteria only)

Product page data example bacterial alignment 02 1
Web-based display of results and interactive alignment tool. Only available for bacteria (yeast and eukaryote are coming soon!)

Ready to sequence?

Send us extracted and purified genomic DNA or cell pellets suspended in Zymo 1X DNA/RNA Shield. We'll handle the rest.

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FAQs

Plasmidsaurus Whole Genome Sequencing is performed using the newest long-read sequencing technology from Oxford Nanopore Technologies (ONT).

We use a transposome complex to construct an amplification-free long-read sequencing library, including minimal fragmentation of the input genomic DNA in a sequence-independent manner, and return you the annotated genome assembly. For more details on our workflows and bioinformatics pipelines, please see technical documentation for Whole Genome Sequencing. 

Our bacterial, yeast, and eukaryotic services offer similar library preparation and sequencing quality but specialized genome extraction services, sequencing depth, and bioinformatics pipelines optimized for bacteria, yeast, and eukaryotes (including filamentous fungi and multicellular animals). 

We recommend choosing the option that matches the organism you are sequencing. If you need more read depth for yeast sequencing, you can submit yeast samples to the Eukaryotic genome services that offer higher data targets. Deeper sequencing is also available for multicellular genomes via our Custom Service

The Hybrid ONT + Illumina option for Bacteria and Yeast Genome Sequencing polishes your long-read ONT assembly with Illumina short reads (paired-end 2x150bp) using the Polypolish v0.6 tool to improve the consensus quality at those tricky homopolymer runs and methylation motifs. 

This Hybrid service is ideal when:

  • You anticipate an abundance of homopolymers and/or methylated motifs in the genome
  • You are planning to use the genome assembly result as a reference for SNP variant calling

The Hybrid service is not generally necessary if your primary goal is to obtain a high quality, high coverage, highly contiguous genome assembly, as contiguity is entirely determined by the quality and quantity of the ONT long read data. Adding in short Illumina reads with the Hybrid service has no impact on the number, size, or topology of assembled chromosomes/elements, and instead only improves nucleotide-level basecalling accuracy in homopolymers and methylated motifs via polishing. For this reason, we do not repeat the genome assembly or contig identification workflows after Illumina polishing.
 
Please note: Hybrid ordering options are not currently available for the Eukaryotic Genome, but you can request to add Illumina data to your ONT eukaryotic data through the Custom Sequencing service.

The Eukaryotic Genome Sequencing service offers four different service levels,  which allow you to select the target amount of output per sample, measured in Gb of fastq data. We don't specify output in number of reads because that varies based on the specific read length distribution in question. 

The Service Level recommendations below are based on a target of approximately 30x genome coverage, which is typically sufficient to generate a high-quality de novo genome assembly with annotations. Depending on your application, you can submit your sample to a different service level than is recommended in order to obtain a different amount of coverage. For example:

  • If you plan to use the raw reads to perform variant analysis against a reference genome, you might need less genome coverage, e.g. 10-20x. Please note that while the assembly is more likely to fail at lower coverage levels, you will still be able to use the raw reads to perform your analysis
  • If you plan to use the raw reads to generate a haplotype-phased de novo genome assembly, in some cases you might need more genome coverage, e.g. 60-100x
Service LevelExample SpeciesApprox. Genome Size (Haploid)Approx. Genome Coverage
1 GbPlasmodium falciparum (protozoan)23 Mb43x
Fusarium oxysporum (fungus)36 Mb28x
Neurospora crassa (fungus)43 Mb23x
5 GbCaenorhabditis elegans (nematode)100 Mb50x
Daphnia pulex (water flea)125 Mb40x
Drosophila melanogaster (fruit fly)180Mb28x
15 GbAnopheles gambiae (mosquito)278 Mb54x
Oryza sativa (rice)430 Mb35x
Mimulus guttatus (monkeyflower)450 Mb30x
50 - 100 Gb (1 flow cell)Danio rerio (zebrafish)1.5 Gb33-66x
Mus musculus (mouse)2.7 Gb18-36x
Homo sapiens (human)3.3 Gb15-30x

The typical turnaround time in business days is listed below for each service. This is the average time it takes to return data to customers who are submitting orders from US, UK and EU. Turnaround times for customers in APAC may be slightly longer due to the time required in transit to our lab in Singapore.

Low sample quality can also negatively impact turnaround time and the quality of your results. Please read sample prep instructions carefully to make sure your samples meet input requirements. Due to variability in shipping logistics and sample quality that is outside our control we cannot guarantee turnaround times or sample success rates.

ServiceFrom pre-extracted gDNAWith extractionHybrid from pre-extracted gDNAHybrid with extraction
Bacteria or yeast6-8 8-10
Eukaryotic 1 Gb & 5 Gb3-55-8N/AN/A
Eukaryotic 15 Gb & Full flow cell

5-8 days for FASTQ data

1-4 weeks for assemblies

8-11 days for FASTQ data

1-4 weeks for assemblies

N/AN/A