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Sample Preparation and Shipping

Microbiome Sample Prep

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Microbiome 16S Amplification & Sequencing with DNA Extraction

Service LevelSample InputRead Depth/Sample
StandardEnvironmental samplesUp to 5,000
BigUp to 10,000
HugeUp to 20,000
BrontoUp to 500,000
01

Weigh your sample and mix with Zymo 1X DNA/RNA shield.

Gather the specified weight or volume of your sample according to the type of source material. 

Source MaterialRequired Amount of Material
Soil100-250 mg
Feces100-250 mg
Swab (e.g. buccal, nasal)1 swab
Filter (e.g. air, water)1 filter, cut into small pieces
Liquid (e.g. water, urine)100-250 uL
Cell culture50-100 mg wet pellet
Biofilm50-100 mg wet biofilm

Add 500 uL of Zymo 1X DNA/RNA Shield into a 2 mL screw cap tube.

Place the specified amount of the source material into the tube containing DNA/RNA Shield. Invert 5-10x to mix.

02

Package your sample securely.

Set up your order online.

Label each screw cap tube with the first two characters of the order code and the sample number. For example, “XX-3” would indicate order XXYYZZ, sample 3.

Samples must be obscured from view. You can use opaque 2mL tubes or wrap standard 2 mL screw cap tubes in foil.

Place the foil wrapped screw cap tubes in a rigid container, such as a falcon tube or tube box.

Double-bag the rigid vessel in at least two plastic bags.

Place an absorbent pad into the plastic bag along with the rigid vessel. For orders of more than 10 samples, please put the tubes in a tube box and load the samples row by row in numerical order.

03

Ship us your sample.

Direct shipping to our Eugene, Oregon lab is required. Dropboxes cannot be used for raw biological materials.

Due to regulations on the shipment of biological samples, we can only accept shipments of environmental samples from labs in the United States.

Place packaged samples in a sturdy cardboard envelope, padded envelope, or cardboard box via any Express or First-Class shipping carrier such as FedEx, UPS, DHL, or USPS. 

Ship samples using the printed label directly to our Eugene, Oregon lab at room temperature. Dry ice and cold packs are not necessary.

Ship to:
Plasmidsaurus
1850 Millrace Drive, Suite 200
Eugene, OR 97403