Abstract:
The perinatal and early postnatal mouse hippocampus is one of the best-studied neurodevelopmental windows, with well-defined gene expression level changes associated with neurogenesis, synaptogenesis, and glial maturation milestones. Yet few contiguous datasets capture these changes in one framework built for cross-time-point comparison.
We developed a low-cost, high-throughput 3′ RNA-Seq method to characterize transcriptional changes from E18.5 to P28. We compare our results to well-described “textbook” developmental milestones and the associated gene expression dynamics as a benchmark.
