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Featured Microbiome 16S / 18S / ITS Resources

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Microbiome Marker Gene Sequencing (16S/18S/ITS) Sample Prep

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Microbiome Marker Gene Sequencing (16S/18S/ITS) Technical Documentation

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Microbiome Marker Gene Sequencing (16S/18S/ITS) FAQ

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How to decide between Microbiome Marker Gene Sequencing (16S/18S/ITS) and Shotgun Metagenomics

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Prep with Plasmidsaurus | How to Sequence Microbiomes Starting From DNA or Amplicons

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Microbial Marker Gene Sequencing (16S/18S/ITS)

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Ambrosia beetles are farmers. They carry fungi in special pockets on their bodies and grow them as food, a partnership that has evolved independently at least a dozen times. But when these beetles spread to new regions, some of their fungal crops become devastating tree killers. Until recently, researchers lacked the genetic tools to study many of them.

In a study published in Fungal Biology and Biotechnology, Joseph et al. (2026) show that genetic tools developed for one ambrosia beetle fungus can be used to genetically modify several of its diverse relatives.

To build these tools, the researchers used Plasmidsaurus ZeroPrep to verify that their plasmids contained the intended sequences.

This work opens the door to studying how these partnerships work, and how some turn harmful.

With its enormous claws, Therizinosaurus looked like a formidable predator. But this dinosaur was likely a herbivore, a reminder that appearances don’t always tell the whole story.

The same can be true for cell cultures.

Mycoplasma can alter cell signaling and gene expression without causing obvious changes in the culture. Everything may look fine while unwanted contamination affects the biology you’re trying to measure.

Your RNA-Seq data can help uncover what isn’t visible. Plasmidsaurus now includes mycoplasma screening with all RNA-Seq results. We analyze non-host reads and screen for nine mycoplasma and related species, looking for strong evidence of contamination.

No extra sample or additional sequencing, just more insight from data you’re already generating.

Is your HEK293 really HEK293?

Cell line misidentification is more common than you might think. The International Cell Line Authentication Committee estimates the worldwide average incidence of misidentified cell line use at 22%.

Now every Plasmidsaurus RNA-Seq order checks for you. We use the SNPs already captured in your RNA-Seq reads to confirm sample identity, so there's no separate assay and no extra sequencing step.

With this approach, Plasmidsaurus can:
- Compare human samples against 917 cataloged cell lines
- Flag potential mixtures when a second measured cell line accounts for 10% or more of reads
- Assign mouse samples to 45 strain background groups

🔗 Link in Bio to the full technical note

More dinosaur species to add to the encyclopedia 🦕

Since 2021, you've sent us your plasmids and woken up to the full sequence. Now we're taking the miniprep off your plate too.

With ZeroPrep colony sequencing, you send us a colony, plate, or overnight culture and get whole plasmid sequences back overnight or even the same day. No miniprep. No PCR. No bias.

With ZeroPrep, you can:
✅ Focus on your hits and stop prepping clones you'll throw away
✅ Get to the results you want up to a day faster
✅ Trust your data with high-quality, amplification-free performance

Which clone are you sequencing first? 🤔

Even lab days need a little downtime 💤

Could the way that bacteria repair DNA help fight antibiotic resistance?

Meet Jolyn Pan, a postdoctoral fellow in the C2 Labs at the University of Waikato. She’s studying how the enzyme LigE helps Neisseria gonorrhoeae repair DNA before potentially acquiring antibiotic resistance and virulence genes from its environment.

With Plasmidsaurus, Jolyn can quickly confirm that the constructs used in her experiments are exactly as intended: “I can just check my sequencing data against my reference gene on the Plasmidsaurus website and it will easily tell me of any mismatches on its website.”

Swipe to learn how Jolyn’s research could uncover new ways to make existing antibiotics more effective against gonorrhoea.

We love seeing your creative interpretations 🦖

But our name is still Plasmidsaurus.

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